5C58

A double mutant of serratia marcescens hemophore receptor HasR in complex with its hemophore HasA and heme

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	HasR protein	polymer	865	94825.6	1	UniProt (Q79AD2) Pfam (PF07660) Pfam (PF07715) Pfam (PF00593)	Serratia marcescens
2	B (B)	Hemophore HasA	polymer	206	21523.2	1	UniProt (Q54450) Pfam (PF06438)	Serratia marcescens	Heme acquisition system protein A
3	C (B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)

Sequence modifications

A: 1 - 865 (UniProt: Q79AD2)

PDB	External Database	Details
Ala 297	Arg 331	engineered mutation
Ala 645	Gly 679	engineered mutation
Ala 800	Asn 834	engineered mutation

B: 2 - 188 (UniProt: Q54450)

PDB	External Database	Details
Met -17	-	initiating methionine
Arg -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Ile -6	-	expression tag
Arg -5	-	expression tag
Met -4	-	expression tag
Arg -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Tyr 0	-	expression tag
Pro 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	116348.8
Non-Polymers*	Number of molecules	1
	Total formula weight	616.5
All*	Total formula weight	116965.3

*Water molecules are not included.