5C58
A double mutant of serratia marcescens hemophore receptor HasR in complex with its hemophore HasA and heme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-06-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 101.971, 114.619, 260.757 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.749 - 2.795 |
R-factor | 0.2496 |
Rwork | 0.247 |
R-free | 0.29640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3csl 1dk0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.667 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10pre-2083_1692: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.960 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmerge | 0.095 | 2.227 |
Number of reflections | 37882 | |
<I/σ(I)> | 13.26 | 0.91 |
Completeness [%] | 99.0 | 94.9 |
Redundancy | 6.3 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100mM Tris pH 7 to 8, 1.8 to 2.2mM NaCl, 100mM K2HPO4 |