5C1S

Crystal structure of the GDP-bound fast hydrolyzing mutant (V71A/K73Q) of EhRabX3 from Entamoeba histolytica

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Small GTPase EhRabX3	polymer	391	45754.7	2	UniProt (Q5NT25)	Entamoeba histolytica
2	C, D (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
3	E, F (A, B)	water	water		18.0	54	Chemie (HOH)

Sequence modifications

A, B: 2 - 349 (UniProt: Q5NT25)

PDB	External Database	Details
Mse -41	-	expression tag
Gly -40	-	expression tag
Ser -39	-	expression tag
Ser -38	-	expression tag
His -37	-	expression tag
His -36	-	expression tag
His -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
Gly -29	-	expression tag
Leu -28	-	expression tag
Val -27	-	expression tag
Pro -26	-	expression tag
Arg -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
His -22	-	expression tag
Mse -21	-	expression tag
Ala -20	-	expression tag
Ser -19	-	expression tag
Mse -18	-	expression tag
Thr -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Gln -14	-	expression tag
Gln -13	-	expression tag
Mse -12	-	expression tag
Gly -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Mse -7	-	expression tag
Ala -6	-	expression tag
Mse -5	-	expression tag
Ile -4	-	expression tag
Arg -3	-	expression tag
Pro -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag
Mse 1	-	expression tag
Ala 71	Val 71	engineered mutation
Gln 73	Lys 73	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	91509.5
Non-Polymers*	Number of molecules	2
	Total formula weight	886.4
All*	Total formula weight	92395.9

*Water molecules are not included.