5BW8
2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | ATPase GET3 | polymer | 373 | 41576.8 | 2 | UniProt (B3LGZ3) Pfam (PF02374) UniProt (by SIFTS) (Q12154) | Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast) | Arsenical pump-driving ATPase,Arsenite-stimulated ATPase,Golgi to ER traffic protein 3,Guided entry of tail-anchored proteins 3 |
| 2 | C (C) | Golgi to ER traffic protein 4 | polymer | 305 | 35803.3 | 1 | UniProt (Q12125) Pfam (PF04190) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Guided entry of tail-anchored proteins 4 |
| 3 | D (D) | Ubiquitin-like protein MDY2 | polymer | 54 | 6034.0 | 1 | UniProt (Q12285) Pfam (PF16843) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Golgi to ER traffic protein 5,Mating-deficient protein 2,Translation machinery-associated protein 24 |
| 4 | E (Z) | Unknown Protein | polymer | 13 | 1124.4 | 1 | Saccharomyces cerevisiae | ||
| 5 | F (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
A, B: 2 - 354 (UniProt: B3LGZ3)
C: 1 - 290 (UniProt: Q12125)
| PDB | External Database | Details |
|---|---|---|
| Met -18 | - | initiating methionine |
| Gly -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Val 0 | - | expression tag |
| Asp 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 258 | Lys 258 | engineered mutation |
| Ala 260 | Lys 260 | engineered mutation |
| Glu 291 | - | expression tag |
| Asn 292 | - | expression tag |
| Leu 293 | - | expression tag |
| Tyr 294 | - | expression tag |
| Phe 295 | - | expression tag |
| Gln 296 | - | expression tag |
| Ser 297 | - | expression tag |
| Leu 298 | - | expression tag |
| Glu 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
| His 302 | - | expression tag |
| His 303 | - | expression tag |
| His 304 | - | expression tag |
| His 305 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 126115.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 126180.8 |
