5BW8

2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ATPase GET3	polymer	373	41576.8	2	UniProt (B3LGZ3) Pfam (PF02374) UniProt (by SIFTS) (Q12154) In PDB	Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast)	Arsenical pump-driving ATPase,Arsenite-stimulated ATPase,Golgi to ER traffic protein 3,Guided entry of tail-anchored proteins 3
2	C	Golgi to ER traffic protein 4	polymer	305	35803.3	1	UniProt (Q12125) Pfam (PF04190) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Guided entry of tail-anchored proteins 4
3	D	Ubiquitin-like protein MDY2	polymer	54	6034.0	1	UniProt (Q12285) Pfam (PF16843) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Golgi to ER traffic protein 5,Mating-deficient protein 2,Translation machinery-associated protein 24
4	Z	Unknown Protein	polymer	13	1124.4	1		Saccharomyces cerevisiae
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A, B: 2 - 354 (UniProt: B3LGZ3)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Val 0	-	expression tag
Asp 1	-	expression tag

C: 1 - 290 (UniProt: Q12125)

PDB	External Database	Details
Ala 258	Lys 258	engineered mutation
Ala 260	Lys 260	engineered mutation
Glu 291	-	expression tag
Asn 292	-	expression tag
Leu 293	-	expression tag
Tyr 294	-	expression tag
Phe 295	-	expression tag
Gln 296	-	expression tag
Ser 297	-	expression tag
Leu 298	-	expression tag
Glu 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	126115.4
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	126180.8

*Water molecules are not included.