5BW8
2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 111.960, 238.050, 52.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.800 - 2.800 |
| R-factor | 0.2259 |
| Rwork | 0.224 |
| R-free | 0.26161 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3A37 and 3LKU |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.697 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0043) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 34114 | |
| <I/σ(I)> | 8.3 | 1.6 |
| Completeness [%] | 97.0 | 95.7 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 17% PEG 3350, 0.24 M Na citrate, and 30 mM TCEP |
