5BW7
Crystal structure of nonfucosylated Fc Y296W mutant complexed with bis-glycosylated soluble form of Fc gamma receptor IIIa
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Ig gamma-1 chain C region | polymer | 223 | 25120.5 | 2 | UniProt (P01857) Pfam (PF07654) | Homo sapiens (Human) | |
| 2 | C (C) | Low affinity immunoglobulin gamma Fc region receptor III-A | polymer | 179 | 20641.0 | 1 | UniProt (P08637) Pfam (PF13895) | Homo sapiens (Human) | CD16a antigen,Fc-gamma RIII-alpha,FcRIIIa,FcR-10,IgG Fc receptor III-2 |
| 3 | D (D) | beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1479.3 | 1 | In PDB GlyTouCan (G89993FE) | |||
| 4 | E (E) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1317.2 | 1 | In PDB GlyTouCan (G39213VZ) | |||
| 5 | F (F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1260.2 | 1 | In PDB GlyTouCan (G45889JQ) | |||
| 6 | G (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 951.9 | 1 | In PDB GlyTouCan (G10133VD) | |||
| 7 | H (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 8 | I, J, K (A, B, C) | water | water | 18.0 | 26 | Chemie (HOH) |
Sequence modifications
A, B: 225 - 447 (UniProt: P01857)
C: 3 - 175 (UniProt: P08637)
| PDB | External Database | Details |
|---|---|---|
| Trp 296 | Tyr 179 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gln 38 | Asn 56 | engineered mutation |
| Gln 74 | Asn 92 | engineered mutation |
| Val 158 | Phe 176 | engineered mutation |
| Gln 169 | Asn 187 | engineered mutation |
| His 176 | - | expression tag |
| His 177 | - | expression tag |
| His 178 | - | expression tag |
| His 179 | - | expression tag |
| His 180 | - | expression tag |
| His 181 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 70881.9 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 5008.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 35.5 | |
| All* | Total formula weight | 75926.0 |
