5BW4

Crystal structure of the 16S rRNA (adenine(1408)-N(1))-methyltransferase W203A mutant with cosubstrate SAM from Catenulisporales acidiphilia

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	16S rRNA (adenine(1408)-N(1))-methyltransferase	polymer	267	28511.5	2	UniProt (C7Q5P8) In PDB	Catenulispora acidiphila
2	A, B	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
3		water	water		18.0	56	Chemie (HOH)

Sequence modifications

A, B: 1 - 250 (UniProt: C7Q5P8)

PDB	External Database	Details
Met -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ala -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 203	Trp 203	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57023.0
Non-Polymers*	Number of molecules	2
	Total formula weight	796.9
All*	Total formula weight	57819.8

*Water molecules are not included.