5BTT

Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein	polymer	230	25854.1	1	UniProt (A0A059PIQ0) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	B (B)	Green fluorescent protein	polymer	230	25854.1	1	UniProt (A0A059PIQ0) Pfam (PF01353)	Aequorea victoria (Jellyfish)
3	C, D, E, F, G... (A, B)	SULFATE ION	non-polymer		96.1	11	Chemie (SO4)
4	H, O (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	P, Q (A, B)	water	water		18.0	265	Chemie (HOH)

Sequence modifications

A: 3 - 231 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Ser 2	-	expression tag
Arg 30	Ser 30	conflict
Cro	Thr 65	chromophore
Cro	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Ser 72	Ala 72	conflict
Arg 80	Gln 80	conflict
4v0 148	His 148	conflict
Val 206	Ala 206	conflict

B: 3 - 231 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Ser 2	-	expression tag
Arg 30	Ser 30	conflict
Cro	Thr 65	chromophore
Cro	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Ser 72	Ala 72	conflict
Arg 80	Gln 80	conflict
Hox 148	His 148	conflict
Val 206	Ala 206	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51708.2
Non-Polymers*	Number of molecules	13
	Total formula weight	1240.9
All*	Total formula weight	52949.0

*Water molecules are not included.