5BTT

Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 43 21 2
Unit cell lengths	135.140, 135.140, 69.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.240 - 2.140
R-factor	0.1707
Rwork	0.169
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.059
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER (2.5.1)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.240	56.240	2.200
High resolution limit [Å]	2.140	9.570	2.140
Rmerge	0.081	0.036	0.714
Rpim	0.022	0.010	0.193
Total number of observations	525718	6469	37806
Number of reflections	36124
<I/σ(I)>	22.9	64.8	4
Completeness [%]	100.0	99.6	100
Redundancy	14.6	13.3	14.4
CC(1/2)	0.999	0.999	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	297	50 mM MMT, 2.5 M (NH4)2SO4