5BT9

Crystal Structure of FolM Alternative dihydrofolate reductase 1 from Brucella canis complexed with NADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	3-oxoacyl-(Acyl-carrier-protein) reductase	polymer	269	29989.2	4	UniProt (A9MA73) Pfam (PF00106)	Brucella canis (strain ATCC 23365 / NCTC 10854)
2	E, F, G, H (A, B, C, D)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	4	Chemie (NAP)
3	I, J, K, L (A, B, C, D)	water	water		18.0	724	Chemie (HOH)

Sequence modifications

A, B, C, D: 9 - 255 (UniProt: A9MA73)

PDB	External Database	Details
Met -8	-	initiating methionine
Ala -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Leu 2	-	expression tag
Asn 3	-	expression tag
Asp 4	-	expression tag
Pro 5	-	expression tag
Glu 6	-	expression tag
Ala 7	-	expression tag
Arg 8	-	expression tag
Glu 256	-	expression tag
Leu 257	-	expression tag
Pro 258	-	expression tag
Asn 259	-	expression tag
Lys 260	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	119956.8
Non-Polymers*	Number of molecules	4
	Total formula weight	2973.6
All*	Total formula weight	122930.4

*Water molecules are not included.