5BR4
E. coli lactaldehyde reductase (FucO) M185C mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Lactaldehyde reductase | polymer | 391 | 41729.5 | 2 | UniProt (P0A9S2) Pfam (PF00465) UniProt (by SIFTS) (P0A9S1) In PDB | Escherichia coli | Propanediol oxidoreductase |
2 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
3 | A, B | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
4 | A, B | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
5 | A, B | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
6 | water | water | 18.0 | 1167 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 383 (UniProt: P0A9S2)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Cys 185 | Met 184 | engineered mutation |
Leu 384 | - | expression tag |
Glu 385 | - | expression tag |
His 386 | - | expression tag |
His 387 | - | expression tag |
His 388 | - | expression tag |
His 389 | - | expression tag |
His 390 | - | expression tag |
His 391 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 83459.0 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 1875.8 | |
All* | Total formula weight | 85334.8 |