5BR4

E. coli lactaldehyde reductase (FucO) M185C mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Lactaldehyde reductase	polymer	391	41729.5	2	UniProt (P0A9S2) Pfam (PF00465) UniProt (by SIFTS) (P0A9S1)	Escherichia coli	Propanediol oxidoreductase
2	C, I (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	D, J (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
4	E, F, K (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	G, H, L, M (A, B)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
6	N, O (A, B)	water	water		18.0	1167	Chemie (HOH)

Sequence modifications

A, B: 2 - 383 (UniProt: P0A9S2)

PDB	External Database	Details
Met 1	-	initiating methionine
Cys 185	Met 184	engineered mutation
Leu 384	-	expression tag
Glu 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	83459.0
Non-Polymers*	Number of molecules	11
	Total formula weight	1875.8
All*	Total formula weight	85334.8

*Water molecules are not included.