5BR4
E. coli lactaldehyde reductase (FucO) M185C mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004022 | molecular_function | alcohol dehydrogenase (NAD+) activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006004 | biological_process | fucose metabolic process |
| A | 0008198 | molecular_function | ferrous iron binding |
| A | 0008912 | molecular_function | lactaldehyde reductase activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019301 | biological_process | rhamnose catabolic process |
| A | 0019317 | biological_process | fucose catabolic process |
| A | 0042355 | biological_process | L-fucose catabolic process |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0042846 | biological_process | glycol catabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0052660 | molecular_function | R-lactaldehyde reductase activity |
| A | 0052661 | molecular_function | S-lactaldehyde reductase activity |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004022 | molecular_function | alcohol dehydrogenase (NAD+) activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006004 | biological_process | fucose metabolic process |
| B | 0008198 | molecular_function | ferrous iron binding |
| B | 0008912 | molecular_function | lactaldehyde reductase activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0019301 | biological_process | rhamnose catabolic process |
| B | 0019317 | biological_process | fucose catabolic process |
| B | 0042355 | biological_process | L-fucose catabolic process |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0042846 | biological_process | glycol catabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0052660 | molecular_function | R-lactaldehyde reductase activity |
| B | 0052661 | molecular_function | S-lactaldehyde reductase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 401 |
| Chain | Residue |
| A | ASP196 |
| A | HIS200 |
| A | HIS263 |
| A | HIS277 |
| site_id | AC2 |
| Number of Residues | 36 |
| Details | binding site for residue NAD A 402 |
| Chain | Residue |
| A | GLY97 |
| A | GLY98 |
| A | SER99 |
| A | PRO100 |
| A | ASP102 |
| A | THR140 |
| A | THR141 |
| A | THR144 |
| A | ALA146 |
| A | THR149 |
| A | ASN151 |
| A | VAL153 |
| A | LYS162 |
| A | MET181 |
| A | GLY184 |
| A | CYS185 |
| A | LEU189 |
| A | HIS277 |
| A | GOL403 |
| A | CL405 |
| A | HOH523 |
| A | HOH559 |
| A | HOH569 |
| A | HOH623 |
| A | HOH672 |
| A | HOH703 |
| A | HOH734 |
| A | HOH770 |
| A | HOH799 |
| A | HOH824 |
| A | HOH837 |
| A | ASP39 |
| A | THR41 |
| A | LEU42 |
| A | PRO70 |
| A | ASN71 |
| site_id | AC3 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 403 |
| Chain | Residue |
| A | ASN151 |
| A | VAL153 |
| A | PHE254 |
| A | LEU259 |
| A | HIS267 |
| A | CYS362 |
| A | NAD402 |
| A | CL405 |
| A | HOH618 |
| A | HOH719 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 404 |
| Chain | Residue |
| A | HIS339 |
| A | ASP342 |
| A | LEU384 |
| A | HOH540 |
| A | HOH567 |
| B | ARG332 |
| B | ASP333 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 405 |
| Chain | Residue |
| A | VAL153 |
| A | HIS267 |
| A | HIS277 |
| A | NAD402 |
| A | GOL403 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for residue CL A 406 |
| Chain | Residue |
| A | LYS162 |
| A | HIS267 |
| A | HIS277 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 401 |
| Chain | Residue |
| B | ASP196 |
| B | HIS200 |
| B | HIS263 |
| B | HIS277 |
| site_id | AC8 |
| Number of Residues | 36 |
| Details | binding site for residue NAD B 402 |
| Chain | Residue |
| B | HOH699 |
| B | HOH715 |
| B | HOH720 |
| B | HOH761 |
| B | HOH779 |
| B | HOH786 |
| B | ASP39 |
| B | THR41 |
| B | LEU42 |
| B | PRO70 |
| B | ASN71 |
| B | GLY97 |
| B | GLY98 |
| B | SER99 |
| B | PRO100 |
| B | ASP102 |
| B | THR140 |
| B | THR141 |
| B | THR144 |
| B | ALA146 |
| B | THR149 |
| B | ASN151 |
| B | VAL153 |
| B | LYS162 |
| B | MET181 |
| B | GLY184 |
| B | CYS185 |
| B | LEU189 |
| B | HIS277 |
| B | GOL403 |
| B | CL405 |
| B | HOH520 |
| B | HOH561 |
| B | HOH578 |
| B | HOH634 |
| B | HOH649 |
| site_id | AC9 |
| Number of Residues | 10 |
| Details | binding site for residue GOL B 403 |
| Chain | Residue |
| B | ASN151 |
| B | VAL153 |
| B | PHE254 |
| B | LEU259 |
| B | HIS267 |
| B | CYS362 |
| B | NAD402 |
| B | CL405 |
| B | HOH574 |
| B | HOH741 |
| site_id | AD1 |
| Number of Residues | 5 |
| Details | binding site for residue CL B 404 |
| Chain | Residue |
| B | LYS162 |
| B | ALA266 |
| B | HIS267 |
| B | HIS277 |
| B | HOH515 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue CL B 405 |
| Chain | Residue |
| B | VAL153 |
| B | HIS267 |
| B | HIS277 |
| B | NAD402 |
| B | GOL403 |
Functional Information from PROSITE/UniProt
| site_id | PS00060 |
| Number of Residues | 21 |
| Details | ADH_IRON_2 Iron-containing alcohol dehydrogenases signature 2. GlVHgmAHpLGAfynTpHGvA |
| Chain | Residue | Details |
| A | GLY260-ALA280 |
| site_id | PS00913 |
| Number of Residues | 29 |
| Details | ADH_IRON_1 Iron-containing alcohol dehydrogenases signature 1. AFiDadmmdgcPpalkAaTgvDALthaiE |
| Chain | Residue | Details |
| A | ALA175-GLU203 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 34 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"2BI4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2BL4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5BR4","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"2BL4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5BR4","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"2BI4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2BL4","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
