5BP9

Crystal structure of SAM-dependent methyltransferase from Bacteroides fragilis in complex with S-Adenosyl-L-homocysteine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Putative methyltransferase protein	polymer	281	32303.6	1	UniProt (Q5LFK0) Pfam (PF08241) In PDB	Bacteroides fragilis
2	A	2-{2-[2-2-(METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL	non-polymer		208.3	1	Chemie (ETE)
3	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
4	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
5		water	water		18.0	335	Chemie (HOH)

Sequence modifications

A: 3 - 260 (UniProt: Q5LFK0)

PDB	External Database	Details
Mse -20	-	initiating methionine
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Gly -11	-	expression tag
Val -10	-	expression tag
Asp -9	-	expression tag
Leu -8	-	expression tag
Gly -7	-	expression tag
Thr -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Ser 1	-	expression tag
Mse 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32303.6
Non-Polymers*	Number of molecules	3
	Total formula weight	786.9
All*	Total formula weight	33090.4

*Water molecules are not included.