5BKF
Cyro-EM structure of human Glycine Receptor alpha2-beta heteromer, Glycine bound, desensitized state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glycine receptor subunit alpha-2 | polymer | 364 | 41676.0 | 4 | UniProt (P23416) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | |
| 2 | E (E) | Glycine receptor subunit beta,Green fluorescent protein | polymer | 702 | 78765.1 | 1 | UniProt (P48167) UniProt (P42212) Pfam (PF02931) Pfam (PF02932) Pfam (PF01353) | Homo sapiens (Human) | |
| 3 | F, G, I, J, L... (A, B, C, D, E) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 9 | Chemie (NAG) | |||
| 4 | H, K, N, Q, S (A, B, C, D, E) | GLYCINE | non-polymer | 75.1 | 5 | Chemie (GLY) |
Sequence modifications
A, B, C, D: 1 - 316 (UniProt: P23416)
A, B, C, D: 382 - 425 (UniProt: P23416)
E: 3 - 333 (UniProt: P48167)
E: 333 - 342 (UniProt: P42212)
E: 342 - 475 (UniProt: P48167)
A, B, C, D: 382 - 425 (UniProt: P23416)
| PDB | External Database | Details |
|---|---|---|
| Gly 378 | - | linker |
| Ser 379 | - | linker |
| Ser 380 | - | linker |
| Gly 381 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly -19 | - | expression tag |
| Val -18 | - | expression tag |
| Ala -17 | - | expression tag |
| Met -16 | - | expression tag |
| Pro -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ala -13 | - | expression tag |
| Glu -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Val -9 | - | expression tag |
| Val -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Glu -4 | - | expression tag |
| Val -3 | - | expression tag |
| Leu -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Gln 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Leu 335 | Phe 64 | engineered mutation |
| Thr 335 | Ser 65 | engineered mutation |
| Lys 341 | Ala 206 | engineered mutation |
| Leu 342 | His 231 | engineered mutation |
| Ser 342 | - | linker |
| Gly 342 | - | linker |
| Ser 342 | - | linker |
| Gly 342 | - | linker |
| Ser 342 | - | linker |
| Gly 342 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 333 | - | linker |
| Gly 333 | - | linker |
| Ser 333 | - | linker |
| Ser 333 | - | linker |
| Ala 333 | - | linker |
| Ala 333 | - | linker |
| Ala 333 | - | linker |
| Val 333 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 245469.1 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 2366.2 | |
| All* | Total formula weight | 247835.3 |
