5B55
Crystal structure of hydrogen sulfide-producing enzyme (Fn1055) D232N mutant in complexed with alpha-aminoacrylate intermediate: lysine-dimethylated form
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Cysteine synthase | polymer | 340 | 38564.1 | 2 | UniProt (Q8REP3) Pfam (PF00291) | Fusobacterium nucleatum subsp. nucleatum strain ATCC 25586 | |
| 2 | C, F (A, B) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 2 | Chemie (PLP) | |||
| 3 | D, G (A, B) | 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid | non-polymer | 316.2 | 2 | Chemie (0JO) | |||
| 4 | E, H (A, B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 5 | I, J (A, B) | water | water | 18.0 | 319 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 336 (UniProt: Q8REP3)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Asn 232 | Asp 232 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 77128.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1338.9 | |
| All* | Total formula weight | 78467.2 |
