5B43
Crystal structure of Acidaminococcus sp. Cpf1 in complex with crRNA and target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cpf1 | polymer | 1310 | 151701.2 | 1 | UniProt (U2UMQ6) Pfam (PF18501) Pfam (PF21918) Pfam (PF18516) Pfam (PF18510) | Acidaminococcus sp. BV3L6 | AsCpf1 |
| 2 | B (B) | RNA (43-MER) | polymer | 43 | 13756.1 | 1 | Acidaminococcus | crRNA | |
| 3 | C (C) | DNA (34-MER) | polymer | 34 | 10443.7 | 1 | Acidaminococcus | Target DNA | |
| 4 | D (D) | DNA (5'-D(*CP*AP*GP*TP*CP*CP*TP*TP*TP*A)-3') | polymer | 10 | 2995.0 | 1 | Acidaminococcus | ||
| 5 | E, F, G (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 6 | H (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 7 | I, J (A, B) | water | water | 18.0 | 25 | Chemie (HOH) |
Sequence modifications
A: 1 - 1307 (UniProt: U2UMQ6)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 178896.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 209.2 | |
| All* | Total formula weight | 179105.3 |
