5B2T
Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Guide RNA | polymer | 81 | 26209.6 | 1 | Streptococcus pyogenes | ||
| 2 | B | CRISPR-associated endonuclease Cas9 | polymer | 1372 | 159076.2 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | Streptococcus pyogenes serotype M1 | SpyCas9 |
| 3 | C | Target DNA | polymer | 28 | 8479.5 | 1 | Streptococcus pyogenes | ||
| 4 | D | Non-target DNA, DNA (5'-D(*TP*GP*CP*GP*AP*TP*TP*G)-3') | polymer | 8 | 2457.6 | 1 | Streptococcus pyogenes | ||
| 5 | A, B | POTASSIUM ION | non-polymer | 39.1 | 10 | Chemie (K) | |||
| 6 | A | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 7 | A, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 8 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 9 | water | water | 18.0 | 608 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 10 | Asp 10 | engineered mutation |
| Leu 80 | Cys 80 | engineered mutation |
| Glu 574 | Cys 574 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
| Val 1135 | Asp 1135 | engineered mutation |
| Arg 1218 | Gly 1218 | engineered mutation |
| Glu 1335 | Arg 1335 | engineered mutation |
| Arg 1337 | Thr 1337 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 196222.9 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 833.3 | |
| All* | Total formula weight | 197056.2 |
