5B2Q
Crystal structure of Francisella novicida Cas9 RHA in complex with sgRNA and target DNA (TGG PAM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1632 | 190946.4 | 1 | UniProt (A0Q5Y3) Pfam (PF21069) Pfam (PF22385) | Francisella tularensis subsp. novicida U112 | |
| 2 | B (B) | Guide RNA | polymer | 94 | 30278.9 | 1 | synthetic construct | ||
| 3 | C (C) | Target DNA | polymer | 30 | 8993.8 | 1 | Francisella tularensis subsp. novicida | ||
| 4 | D (D) | DNA (5'-D(*TP*GP*GP*TP*AP*TP*CP*GP*G)-3') | polymer | 9 | 2786.8 | 1 | Francisella tularensis subsp. novicida | ||
| 5 | E (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | F, G, HA, IA (A, B) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 7 | H, I (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 8 | J, JA, K, KA, L... (A, B) | CALCIUM ION | non-polymer | 40.1 | 17 | Chemie (CA) | |||
| 9 | AA, AB, BA, BB, CA... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 24 | Chemie (EDO) | |||
| 10 | FA, GA (A) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 11 | CB, DB, EB, FB (A, B, C, D) | water | water | 18.0 | 993 | Chemie (HOH) |
Sequence modifications
A: 1 - 1629 (UniProt: A0Q5Y3)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 995 | Asn 995 | engineered mutation |
| Arg 1369 | Glu 1369 | conflict |
| His 1449 | Glu 1449 | conflict |
| Ala 1556 | Arg 1556 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 233005.9 | |
| Non-Polymers* | Number of molecules | 50 |
| Total formula weight | 2517.3 | |
| All* | Total formula weight | 235523.2 |
