5B04
Crystal structure of the eukaryotic translation initiation factor 2B from Schizosaccharomyces pombe
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Translation initiation factor eIF-2B subunit alpha | polymer | 341 | 37817.3 | 2 | UniProt (Q9USP0) Pfam (PF01008) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | eIF-2B GDP-GTP exchange factor subunit alpha |
| 2 | C, D (C, D) | Probable translation initiation factor eIF-2B subunit beta | polymer | 399 | 43897.9 | 2 | UniProt (Q9UT76) Pfam (PF01008) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | eIF-2B GDP-GTP exchange factor subunit beta |
| 3 | E, F (E, F) | Probable translation initiation factor eIF-2B subunit gamma | polymer | 458 | 50551.9 | 2 | UniProt (P56288) Pfam (PF00483) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | eIF-2B GDP-GTP exchange factor subunit gamma |
| 4 | G, H (G, H) | Probable translation initiation factor eIF-2B subunit delta | polymer | 467 | 51652.2 | 2 | UniProt (Q09924) Pfam (PF01008) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | eIF-2B GDP-GTP exchange factor subunit delta |
| 5 | I, J (I, J) | Probable translation initiation factor eIF-2B subunit epsilon | polymer | 678 | 76413.5 | 2 | UniProt (P56287) Pfam (PF00483) Pfam (PF00132) Pfam (PF02020) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | eIF-2B GDP-GTP exchange factor subunit epsilon |
| 6 | K, L, M, N, O... (A, B, C, D, G...) | PHOSPHATE ION | non-polymer | 95.0 | 9 | Chemie (PO4) |
Sequence modifications
C, D: 1 - 393 (UniProt: Q9UT76)
E, F: 1 - 458 (UniProt: P56288)
| PDB | External Database | Details |
|---|---|---|
| Gly -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Ile -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Phe 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Tyr 157 | Ile 157 | engineered mutation |
| Thr 158 | Tyr 158 | engineered mutation |
| Val 159 | Gly 159 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 520665.6 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 854.7 | |
| All* | Total formula weight | 521520.3 |
