5AZJ

Crystal structure of glycerol kinase from Trypanosoma brucei gambiense complexed with 4NP (with disulfide bridge)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Glycerol kinase	polymer	518	57064.6	2	UniProt (D3KVM3) Pfam (PF00370) Pfam (PF02782)	Trypanosoma brucei gambiense
2	B, D (B, D)	Glycerol kinase	polymer	518	57144.6	2	UniProt (D3KVM3) Pfam (PF00370) Pfam (PF02782)	Trypanosoma brucei gambiense
3	E, H (A, C)	4-NITROPHENYL PHOSPHATE	non-polymer		219.1	2	Chemie (4NP)
4	F, G, I, J (A, B, C, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	K, L, M, N (A, B, C, D)	water	water		18.0	102	Chemie (HOH)

Sequence modifications

A, C: 1 - 512 (UniProt: D3KVM3)

PDB	External Database	Details
Gly -5	-	expression tag
Ile -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

B, D: 1 - 512 (UniProt: D3KVM3)

PDB	External Database	Details
Gly -5	-	expression tag
Ile -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	228418.5
Non-Polymers*	Number of molecules	6
	Total formula weight	806.6
All*	Total formula weight	229225.0

*Water molecules are not included.