5AZ8
Crystal structure of MBP-Tom20 fusion protein tethered with ALDH presequence via a disulfide bond
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Maltose-binding periplasmic protein,Mitochondrial import receptor subunit TOM20 homolog | polymer | 435 | 48044.6 | 1 | UniProt (P0AEX9) UniProt (Q62760) Pfam (PF01547) Pfam (PF02064) | Escherichia coli (strain K12) | MBP,MMBP,Maltodextrin-binding protein,Mitochondrial 20 kDa outer membrane protein,Outer mitochondrial membrane receptor Tom20 |
| 2 | B (B) | peptide GPRLSRLLSYAGC | polymer | 13 | 1393.7 | 1 | UniProt (by SIFTS) (P11884) | Rattus norvegicus | |
| 3 | C (C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | D (B) | ACETYLAMINO-ACETIC ACID | non-polymer | 117.1 | 1 | Chemie (AAC) | |||
| 5 | E, F (A, B) | water | water | 18.0 | 403 | Chemie (HOH) |
Sequence modifications
A: 3 - 370 (UniProt: P0AEX9)
A: 375 - 436 (UniProt: Q62760)
| PDB | External Database | Details |
|---|---|---|
| Met 2 | - | initiating methionine |
| Val 314 | Ala 338 | engineered mutation |
| Lys 371 | - | linker |
| Glu 372 | - | linker |
| Ala 373 | - | linker |
| Leu 374 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 49438.3 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 117.1 | |
| All* | Total formula weight | 49897.7 |
