5AZ8
Crystal structure of MBP-Tom20 fusion protein tethered with ALDH presequence via a disulfide bond
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 69.013, 69.013, 212.604 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.110 - 1.700 |
R-factor | 0.16347 |
Rwork | 0.162 |
R-free | 0.19555 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1anf 2v1t |
RMSD bond length | 0.021 |
RMSD bond angle | 2.010 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 56782 |
<I/σ(I)> | 22.1 |
Completeness [%] | 98.4 |
Redundancy | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% PEG 8000, 20% Glycerol, 0.04M Potassium thiocyanate |