5AX8

Recombinant expression, purification and preliminary crystallographic studies of the mature form of human mitochondrial aspartate aminotransferase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Aspartate aminotransferase, mitochondrial	polymer	407	45583.1	4	UniProt (P00505) Pfam (PF00155) In PDB	Homo sapiens (Human)	mAspAT,Fatty acid-binding protein,FABP-1,Glutamate oxaloacetate transaminase 2,Kynurenine aminotransferase 4,Kynurenine aminotransferase IV,Kynurenine--oxoglutarate transaminase 4,Kynurenine--oxoglutarate transaminase IV,Plasma membrane-associated fatty acid-binding protein,FABPpm,Transaminase A

Sequence modifications

A, B, C, D: 30 - 430 (UniProt: P00505)

PDB	External Database	Details
Gly 346	Val 346	engineered mutation
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	182332.3
All*	Total formula weight	182332.3

*Water molecules are not included.