5APU

Sequence IANKEDKAD inserted between GCN4 adaptors - Structure A9b black

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	GENERAL CONTROL PROTEIN GCN4	polymer	95	11394.1	3	UniProt (P03069) Pfam (PF07716) In PDB	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	AMINO ACID BIOSYNTHESIS REGULATORY PROTEIN
2	A	UREA	non-polymer		60.1	1	Chemie (URE)
3		water	water		18.0	216	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 28 (UniProt: P03069)

PDB	External Database	Details
Met -25	-	expression tag
Lys -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Pro -17	-	expression tag
Met -16	-	expression tag
Ser -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Val 15	Asn 264	engineered mutation

A, B, C: 38 - 69 (UniProt: P03069)

PDB	External Database	Details
Ile 29	-	linker
Ala 30	-	linker
Asn 31	-	linker
Lys 32	-	linker
Glu 33	-	linker
Asp 34	-	linker
Lys 35	-	linker
Ala 36	-	linker
Asp 37	-	linker
Val 52	Asn 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	34182.2
Non-Polymers*	Number of molecules	1
	Total formula weight	60.1
All*	Total formula weight	34242.3

*Water molecules are not included.