5AIU

A complex of RNF4-RING domain, Ubc13-Ub (isopeptide crosslink)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	E3 UBIQUITIN-PROTEIN LIGASE RNF4	polymer	133	14758.2	1	UniProt (O88846) Pfam (PF13639)	RATTUS NORVEGICUS (NORWAY RAT)	RING FINGER PROTEIN 4, SMALL NUCLEAR RING FINGER PROTEIN, PROTEIN SNURF, "RING DOMAIN, UBC13"
2	B, D (B, E)	UBIQUITIN-CONJUGATING ENZYME E2 N	polymer	154	17253.8	2	UniProt (P61088) Pfam (PF00179)	HOMO SAPIENS (HUMAN)	BENDLESS-LIKE UBIQUITIN-CONJUGATING ENZYME, UBC13, UBCH13, UBIQUITIN CARRIER PROTEIN N, UBIQUITIN-PROTEIN LIGASE N, UBC13
3	C, E (C, F)	POLYUBIQUITIN-C	polymer	76	8576.8	2	UniProt (P0CG48) Pfam (PF00240)	HOMO SAPIENS (HUMAN)
4	F, G, H, I (A)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	J, K, L, M, N... (A, B, C, E, F)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
6	P, Q, R, S, T (A, B, C, E, F)	water	water		18.0	33	Chemie (HOH)

Sequence modifications

A: 131 - 194 (UniProt: O88846)

PDB	External Database	Details
Gly 127	-	expression tag
Ala 128	-	expression tag
Met 129	-	expression tag
Gly 130	-	expression tag

A: 196 - 259 (UniProt: O88846)

PDB	External Database	Details
Gly 195	-	linker

B, E: 1 - 152 (UniProt: P61088)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Lys 87	Cys 87	engineered mutation
Ala 92	Lys 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	66419.5
Non-Polymers*	Number of molecules	10
	Total formula weight	634.0
All*	Total formula weight	67053.5

*Water molecules are not included.