5AIU
A complex of RNF4-RING domain, Ubc13-Ub (isopeptide crosslink)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-24 |
| Detector | RIGAKU SATURN |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.190, 169.210, 52.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.020 - 2.210 |
| R-factor | 0.21857 |
| Rwork | 0.217 |
| R-free | 0.25541 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.696 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 2.260 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.060 | 0.150 |
| Number of reflections | 29485 | |
| <I/σ(I)> | 20.9 | 7.8 |
| Completeness [%] | 95.0 | 74.8 |
| Redundancy | 5.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1,2-ETHANEDIOL |
