5AA1

Crystal structure of MltF from Pseudomonas aeruginosa in complex with NAG-anhNAM-pentapeptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	MEMBRANE-BOUND LYTIC MUREIN TRANSGLYCOSYLASE F	polymer	499	56270.5	4	UniProt (Q9HXN1) Pfam (PF00497) Pfam (PF01464)	PSEUDOMONAS AERUGINOSA	MUREIN LYASE F
2	E (E)	N-ACETYLGLUCOSAMINE-1,6-ANHYDRO-N-ACETYLMURAMIC ACID L-ALA-D-GLU-M-DAP-D-ALA-D-ALA	polymer	6	789.8	1		PSEUDOMONAS AERUGINOSA
3	F, G, H, I, J (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
4	K, L, M, N (A, B, C, D)	water	water		18.0	129	Chemie (HOH)

Sequence modifications

A, B, C, D: 8 - 490 (UniProt: Q9HXN1)

PDB	External Database	Details
Met -8	-	expression tag
Ala -7	-	expression tag
Pro -6	-	expression tag
Ser -5	-	expression tag
Arg -4	-	expression tag
Leu -3	-	expression tag
Cys -2	-	expression tag
Val -1	-	expression tag
Tyr 0	-	expression tag
Cys 1	-	expression tag
Ala 2	-	expression tag
Asp 3	-	expression tag
Val 4	-	expression tag
Cys 5	-	expression tag
Pro 6	-	expression tag
Asp 7	-	expression tag
Thr 281	Ala 281	conflict
Lys 302	Leu 302	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	225872.0
Non-Polymers*	Number of molecules	5
	Total formula weight	177.3
All*	Total formula weight	226049.2

*Water molecules are not included.