5A74

Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Mn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA ENDONUCLEASE I-CVUI	polymer	172	19292.5	2	UniProt (P56347) Pfam (PF00961)	CHLORELLA VULGARIS	I-CVUI, 23S RRNA INTRON PROTEIN
2	C, D (C, D)	14MER DNA, 5'-D(*TP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP)-3'	polymer	14	4264.8	2		SYNTHETIC CONSTRUCT
3	E, F (E, F)	10MER DNA, 5'-D(*GP*AP*CP*GP*TP*TP*CP*TP*GP*AP)-3'	polymer	10	3060.0	2		SYNTHETIC CONSTRUCT
4	G, H, I (E, F)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
5	J, K, L, M, N... (A, B, C, D, E...)	water	water		18.0	205	Chemie (HOH)

Sequence modifications

A, B: 3 - 162 (UniProt: P56347)

PDB	External Database	Details
Ala 2	-	expression tag
Ser 163	-	expression tag
Ala 164	-	expression tag
Ala 165	-	expression tag
Leu 166	-	expression tag
Glu 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
Gln 54	Arg 53	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	53234.7
Non-Polymers*	Number of molecules	3
	Total formula weight	164.8
All*	Total formula weight	53399.5

*Water molecules are not included.