5A74
Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Mn
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA ENDONUCLEASE I-CVUI | polymer | 172 | 19292.5 | 2 | UniProt (P56347) Pfam (PF00961) In PDB | CHLORELLA VULGARIS | I-CVUI, 23S RRNA INTRON PROTEIN |
2 | C, D | 14MER DNA, 5'-D(*TP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP)-3' | polymer | 14 | 4264.8 | 2 | SYNTHETIC CONSTRUCT | ||
3 | E, F | 10MER DNA, 5'-D(*GP*AP*CP*GP*TP*TP*CP*TP*GP*AP)-3' | polymer | 10 | 3060.0 | 2 | SYNTHETIC CONSTRUCT | ||
4 | E, F | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
5 | water | water | 18.0 | 205 | Chemie (HOH) |
Sequence modifications
A, B: 3 - 162 (UniProt: P56347)
PDB | External Database | Details |
---|---|---|
Ala 2 | - | expression tag |
Ser 163 | - | expression tag |
Ala 164 | - | expression tag |
Ala 165 | - | expression tag |
Leu 166 | - | expression tag |
Glu 167 | - | expression tag |
His 168 | - | expression tag |
His 169 | - | expression tag |
His 170 | - | expression tag |
His 171 | - | expression tag |
His 172 | - | expression tag |
His 173 | - | expression tag |
Gln 54 | Arg 53 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 53234.7 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 164.8 | |
All* | Total formula weight | 53399.5 |