5A74
Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Mn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.730, 82.030, 94.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.944 - 2.500 |
R-factor | 0.1912 |
Rwork | 0.186 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g9y |
RMSD bond length | 0.008 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.630 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.140 | 0.480 |
Number of reflections | 17475 | |
<I/σ(I)> | 6.2 | 2.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |