5A4M
Mechanism of Hydrogen activation by NiFe-hydrogenases
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (L, M) | HYDROGENASE-1 LARGE CHAIN | polymer | 582 | 64751.4 | 2 | UniProt (P0ACD8) Pfam (PF00374) | ESCHERICHIA COLI STR. K-12 SUBSTR. MC4100 | HYD1, MEMBRANE-BOUND HYDROGENASE 1 LARGE SUBUNIT, NIFE HYDROGENASE |
| 2 | C, D (S, T) | HYDROGENASE-1 SMALL CHAIN | polymer | 278 | 30911.2 | 2 | UniProt (P69739) Pfam (PF01058) Pfam (PF14720) | ESCHERICHIA COLI STR. K-12 SUBSTR. MC4100 | HYD1, MEMBRANE-BOUND HYDROGENASE 1 SMALL SUBUNIT, NIFE HYDROGENASE |
| 3 | E, G (L, M) | NI-FE OXIDIZED ACTIVE CENTER | non-polymer | 210.6 | 2 | Chemie (NFV) | |||
| 4 | F, H (L, M) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | I, M (S, T) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 6 | J, N (S, T) | FE3-S4 CLUSTER | non-polymer | 295.8 | 2 | Chemie (F3S) | |||
| 7 | K, O (S, T) | FE4-S3 CLUSTER | non-polymer | 319.6 | 2 | Chemie (SF3) | |||
| 8 | L, P (S, T) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 9 | Q, R, S, T (L, M, S, T) | water | water | 18.0 | 1492 | Chemie (HOH) |
Sequence modifications
S, T: 1 - 266 (UniProt: P69739)
| PDB | External Database | Details |
|---|---|---|
| Gly 267 | - | expression tag |
| Ser 268 | - | expression tag |
| Phe 269 | - | expression tag |
| Tyr 270 | - | expression tag |
| Ser 271 | - | expression tag |
| Arg 272 | - | expression tag |
| His 273 | - | expression tag |
| His 274 | - | expression tag |
| His 275 | - | expression tag |
| His 276 | - | expression tag |
| His 277 | - | expression tag |
| His 278 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 191325.2 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2474.7 | |
| All* | Total formula weight | 193799.9 |
