5A4M

Mechanism of Hydrogen activation by NiFe-hydrogenases

Functional Information from GO Data

Chain	GOid	namespace	contents
L	0005515	molecular_function	protein binding
L	0005886	cellular_component	plasma membrane
L	0006113	biological_process	fermentation
L	0008901	molecular_function	ferredoxin hydrogenase activity
L	0009055	molecular_function	electron transfer activity
L	0009061	biological_process	anaerobic respiration
L	0009267	biological_process	cellular response to starvation
L	0016020	cellular_component	membrane
L	0016151	molecular_function	nickel cation binding
L	0016491	molecular_function	oxidoreductase activity
L	0019645	biological_process	anaerobic electron transport chain
L	0030288	cellular_component	outer membrane-bounded periplasmic space
L	0033748	molecular_function	hydrogenase (acceptor) activity
L	0044569	cellular_component	[Ni-Fe] hydrogenase complex
L	0046872	molecular_function	metal ion binding
L	0098567	cellular_component	periplasmic side of plasma membrane
L	1902421	biological_process	hydrogen metabolic process
M	0005515	molecular_function	protein binding
M	0005886	cellular_component	plasma membrane
M	0006113	biological_process	fermentation
M	0008901	molecular_function	ferredoxin hydrogenase activity
M	0009055	molecular_function	electron transfer activity
M	0009061	biological_process	anaerobic respiration
M	0009267	biological_process	cellular response to starvation
M	0016020	cellular_component	membrane
M	0016151	molecular_function	nickel cation binding
M	0016491	molecular_function	oxidoreductase activity
M	0019645	biological_process	anaerobic electron transport chain
M	0030288	cellular_component	outer membrane-bounded periplasmic space
M	0033748	molecular_function	hydrogenase (acceptor) activity
M	0044569	cellular_component	[Ni-Fe] hydrogenase complex
M	0046872	molecular_function	metal ion binding
M	0098567	cellular_component	periplasmic side of plasma membrane
M	1902421	biological_process	hydrogen metabolic process
S	0008901	molecular_function	ferredoxin hydrogenase activity
S	0009375	cellular_component	ferredoxin hydrogenase complex
S	0051536	molecular_function	iron-sulfur cluster binding
T	0008901	molecular_function	ferredoxin hydrogenase activity
T	0009375	cellular_component	ferredoxin hydrogenase complex
T	0051536	molecular_function	iron-sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE NFV L 601

Chain	Residue
L	CYS76
L	PRO531
L	THR532
L	CYS576
L	CYS579
L	HOH2106
L	CYS79
L	VAL82
L	HIS83
L	ALA507
L	PRO508
L	ARG509
L	LEU512
L	VAL530

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG L 602

Chain	Residue
L	GLU57
L	CYS528
L	HIS582
L	HOH2075
L	HOH2076
L	HOH2396

---

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE NFV M 603

Chain	Residue
M	CYS76
M	CYS79
M	VAL82
M	HIS83
M	ALA507
M	PRO508
M	ARG509
M	LEU512
M	VAL530
M	PRO531
M	THR532
M	CYS576
M	CYS579

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG M 604

Chain	Residue
M	GLU57
M	CYS528
M	HIS582
M	HOH2068
M	HOH2069
M	HOH2390

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SF4 S 401

Chain	Residue
S	HIS187
S	CYS190
S	ARG193
S	CYS215
S	LEU216
S	CYS221

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE F3S S 402

Chain	Residue
L	LYS226
S	THR226
S	ASN228
S	CYS230
S	TRP235
S	CYS249
S	LEU250
S	CYS252

---

site_id	AC7
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SF3 S 404

Chain	Residue
L	HOH2282
S	CYS17
S	CYS19
S	CYS20
S	THR114
S	CYS115
S	CYS120
S	GLY148
S	CYS149
S	HOH2009

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL S 408

Chain	Residue
S	TRP118
S	CYS120
S	GLY256
S	HOH2083

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SF4 T 401

Chain	Residue
T	HIS187
T	CYS190
T	ARG193
T	CYS215
T	LEU216
T	CYS221

---

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE F3S T 402

Chain	Residue
M	LYS226
T	ASN228
T	CYS230
T	TRP235
T	CYS249
T	LEU250
T	CYS252

---

site_id	BC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SF3 T 404

Chain	Residue
M	HOH2265
T	CYS17
T	CYS19
T	CYS20
T	THR114
T	CYS115
T	CYS120
T	GLY148
T	CYS149
T	HOH2009

---

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL T 409

Chain	Residue
T	CYS120
T	GLY256
T	HOH2074

---

Functional Information from PROSITE/UniProt

site_id	PS00507
Number of Residues	26
Details	NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEiilqgrdprdawafvERiCGVC

Chain	Residue	Details
L	ARG54-CYS79

---

site_id	PS00508
Number of Residues	10
Details	NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCLACst.H

Chain	Residue	Details
L	PHE573-HIS582

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	22
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P21853

Chain	Residue	Details
S	CYS17
S	CYS249
S	CYS252
T	CYS17
T	CYS20
T	CYS115
T	CYS149
T	HIS187
T	CYS190
T	CYS215
T	CYS221
S	CYS20
T	CYS230
T	CYS249
T	CYS252
S	CYS115
S	CYS149
S	HIS187
S	CYS190
S	CYS215
S	CYS221
S	CYS230

---