5A1N

The crystal structure of the GST-like domains complex of EPRS-AIMP2 mutant S156D

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE	polymer	175	19310.7	1	UniProt (P07814) Pfam (PF21972)	HOMO SAPIENS (HUMAN)	BIFUNCTIONAL AMINOACYL-TRNA SYNTHETASE, CELL PROLIFERATION-INDUCING GENE 32 PROTEIN, GLUTAMATYL-PROLYL-TRNA SYNTHETASE, GLUTAMYL-TRNA SYNTHETASE, GLURS, PROLYL-TRNA SYNTHETASE, GLUTAMYL-PROLY L-TRNA SYNTHETASE
2	B (B)	AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 2	polymer	240	26818.9	1	UniProt (Q13155) Pfam (PF18569) Pfam (PF00043)	HOMO SAPIENS (HUMAN)	MULTISYNTHASE COMPLEX AUXILIARY COMPONENT P38, PROTEIN JTV-1, AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN
3	C, D (B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	E, F (A, B)	water	water		18.0	199	Chemie (HOH)

Sequence modifications

A: 1 - 175 (UniProt: P07814)

PDB	External Database	Details
Ser 92	Cys 92	engineered mutation
Ser 105	Cys 105	engineered mutation
Ser 123	Cys 123	engineered mutation

B: 90 - 320 (UniProt: Q13155)

PDB	External Database	Details
Met 89	-	expression tag
Leu 321	-	expression tag
Glu 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
Asp 156	Ser 156	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	46129.6
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	46313.8

*Water molecules are not included.