5A1N
The crystal structure of the GST-like domains complex of EPRS-AIMP2 mutant S156D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 75.619, 75.619, 175.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.323 - 2.100 |
| R-factor | 0.1958 |
| Rwork | 0.193 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TO BE PUBLISHED |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.047 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 30430 | |
| <I/σ(I)> | 41 | 4.8 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 12.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 17% PEG 3350, 0.1M BIS-TRIS PH 5.5, 2MM DTT |
