5A0X

Substrate peptide-bound structure of metalloprotease Zmp1 variant E143AY178F from Clostridium difficile

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ZINC METALLOPROTEASE ZMP1	polymer	198	21909.6	2	UniProt (Q183R7) Pfam (PF07737) In PDB	CLOSTRIDIUM DIFFICILE
2	C, D	SUBSTRATE PEPTIDE	polymer	9	774.9	2		CLOSTRIDIUM DIFFICILE
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4		water	water		18.0	428	Chemie (HOH)

Sequence modifications

A, B: 27 - 220 (UniProt: Q183R7)

PDB	External Database	Details
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag
Ala 143	Glu 143	engineered mutation
Phe 178	Tyr 178	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45369.1
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	45499.9

*Water molecules are not included.