5A0X
Substrate peptide-bound structure of metalloprotease Zmp1 variant E143AY178F from Clostridium difficile
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-26 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.435, 42.941, 123.244 |
Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
Resolution | 40.850 - 1.700 |
R-factor | 0.181 |
Rwork | 0.180 |
R-free | 0.20820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a0p |
RMSD bond length | 0.006 |
RMSD bond angle | 0.932 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (in) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.900 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.120 | 0.410 |
Number of reflections | 43083 | |
<I/σ(I)> | 9.58 | 2.78 |
Completeness [%] | 97.8 | 87.8 |
Redundancy | 6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 293 | 2.25 AMMONIUM PHOSPHATE DIBASIC, 0.1 M TRIS PH 9.0 GROWN AT 293K |