4ZXF

Crystal Structure of a Soluble Variant of Monoglyceride Lipase from Saccharomyces Cerevisiae in Complex with a Substrate Analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Monoglyceride lipase	polymer	340	38905.1	4	UniProt (P28321) Pfam (PF12146)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	MGL,Monoacylglycerol hydrolase,MGH,Monoacylglycerol lipase,MAGL,Serine hydrolase YJU3
2	E, F (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	G (C)	1-{3-[(R)-hydroxy(octadecyloxy)phosphoryl]propyl}triaza-1,2-dien-2-ium	non-polymer		418.6	1	Chemie (4S7)
4	H, I (D)	NITRATE ION	non-polymer		62.0	2	Chemie (NO3)
5	J, K, L, M (A, B, C, D)	water	water		18.0	151	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 313 (UniProt: P28321)

PDB	External Database	Details
Met -26	-	initiating methionine
Ser -25	-	expression tag
Tyr -24	-	expression tag
Tyr -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Thr -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 175	Leu 175	engineered mutation
Arg 264	Gln 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	155620.5
Non-Polymers*	Number of molecules	5
	Total formula weight	734.7
All*	Total formula weight	156355.2

*Water molecules are not included.