4ZXF
Crystal Structure of a Soluble Variant of Monoglyceride Lipase from Saccharomyces Cerevisiae in Complex with a Substrate Analog
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Monoglyceride lipase | polymer | 340 | 38905.1 | 4 | UniProt (P28321) Pfam (PF12146) In PDB | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | MGL,Monoacylglycerol hydrolase,MGH,Monoacylglycerol lipase,MAGL,Serine hydrolase YJU3 |
2 | A, B | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
3 | C | 1-{3-[(R)-hydroxy(octadecyloxy)phosphoryl]propyl}triaza-1,2-dien-2-ium | non-polymer | 418.6 | 1 | Chemie (4S7) | |||
4 | D | NITRATE ION | non-polymer | 62.0 | 2 | Chemie (NO3) | |||
5 | water | water | 18.0 | 151 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 313 (UniProt: P28321)
PDB | External Database | Details |
---|---|---|
Met -26 | - | initiating methionine |
Ser -25 | - | expression tag |
Tyr -24 | - | expression tag |
Tyr -23 | - | expression tag |
His -22 | - | expression tag |
His -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
Asp -16 | - | expression tag |
Tyr -15 | - | expression tag |
Asp -14 | - | expression tag |
Ile -13 | - | expression tag |
Pro -12 | - | expression tag |
Thr -11 | - | expression tag |
Thr -10 | - | expression tag |
Glu -9 | - | expression tag |
Asn -8 | - | expression tag |
Leu -7 | - | expression tag |
Tyr -6 | - | expression tag |
Phe -5 | - | expression tag |
Gln -4 | - | expression tag |
Gly -3 | - | expression tag |
Ala -2 | - | expression tag |
Met -1 | - | expression tag |
Gly 0 | - | expression tag |
Ser 175 | Leu 175 | engineered mutation |
Arg 264 | Gln 264 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 155620.5 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 734.7 | |
All* | Total formula weight | 156355.2 |