4ZT0
Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | CRISPR-associated endonuclease Cas9 | polymer | 1369 | 159804.6 | 2 | UniProt (A0A0C6FZC2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) UniProt (by SIFTS) (Q99ZW2) | Streptococcus pyogenes | Csn1 protein, Cas9, CRISPR-Cas9 |
| 2 | B, D (B, D) | single-guide RNA | polymer | 85 | 27501.4 | 2 | Streptococcus pyogenes |
Sequence modifications
A, C: 2 - 1368 (UniProt: A0A0C6FZC2)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Mse 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 374612.0 | |
| All* | Total formula weight | 374612.0 |
