4ZT0

Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	103.238, 143.002, 294.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.153 - 2.900
R-factor	0.2395
Rwork	0.238
R-free	0.26370
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.554
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.700	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.035	0.456
Number of reflections	97000
<I/σ(I)>	12.9	1.7
Completeness [%]	99.4	96.7
Redundancy	9.8	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12% w/v PEG8000, 100 mM sodium cacodylate, pH 6.0, 250 mM sodium chloride