4ZRS

Crystal structure of a cloned feruloyl esterase from a soil metagenomic library

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Esterase	polymer	306	33453.8	2	UniProt (E7DJY5)	uncultured bacterium	feruloyl esterase
2	C, D (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	E, F (A, B)	water	water		18.0	615	Chemie (HOH)

Sequence modifications

A, B: 1 - 290 (UniProt: E7DJY5)

PDB	External Database	Details
Met -15	-	expression tag
Gly -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ile 27	Thr 27	engineered mutation
Leu 84	Ser 84	engineered mutation
Ile 161	Val 161	engineered mutation
Leu 195	His 195	engineered mutation
Cys 243	Gly 243	engineered mutation
Val 259	Ala 259	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66907.5
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	67091.7

*Water molecules are not included.