4ZRS
Crystal structure of a cloned feruloyl esterase from a soil metagenomic library
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-09 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.156, 101.790, 69.822 |
| Unit cell angles | 90.00, 110.62, 90.00 |
Refinement procedure
| Resolution | 65.350 - 2.000 |
| R-factor | 0.15247 |
| Rwork | 0.150 |
| R-free | 0.19930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nsp |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.740 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.350 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.104 | 0.435 |
| Number of reflections | 45906 | |
| <I/σ(I)> | 10.6 | 3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.2 M ammonium sulfate, 100 mM HEPES |
