4ZO7

Crystal structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with gentiobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lin1840 protein	polymer	731	80572.0	2	UniProt (Q92AS9) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310) In PDB	Listeria innocua serovar 6a (strain CLIP 11262)	Beta-glucosidase
2	C	beta-D-glucopyranose-(1-6)-alpha-D-glucopyranose	branched		342.3	1	In PDB GlyTouCan (G91215DP)
3	A	beta-D-glucopyranose	non-polymer		180.2	3	Chemie (BGC)
4	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	A	alpha-D-glucopyranose	non-polymer		180.2	1	Chemie (GLC)
7		water	water		18.0	807	Chemie (HOH)

Sequence modifications

A, B: 1 - 723 (UniProt: Q92AS9)

PDB	External Database	Details
Glu 2	Lys 2	engineered mutation
Ala 270	Asp 270	engineered mutation
Leu 724	-	expression tag
Glu 725	-	expression tag
His 726	-	expression tag
His 727	-	expression tag
His 728	-	expression tag
His 729	-	expression tag
His 730	-	expression tag
His 731	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	161144.1
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	7
	Total formula weight	861.3
All*	Total formula weight	162347.7

*Water molecules are not included.