4ZFK

Ergothioneine-biosynthetic Ntn hydrolase EgtC with glutamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Amidohydrolase EgtC	polymer	235	25240.5	4	UniProt (A0R5M9) Pfam (PF13230) In PDB	Mycobacterium smegmatis
2	C, D, A, B	1,2-ETHANEDIOL	non-polymer		62.1	27	Chemie (EDO)
3	C, A, D, B	GLUTAMINE	non-polymer		146.1	4	Chemie (GLN)
4		water	water		18.0	1153	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 227 (UniProt: A0R5M9)

PDB	External Database	Details
Asp 53	Glu 53	engineered mutation
Val 84	Leu 84	engineered mutation
Leu 137	Val 137	engineered mutation
Arg 188	His 188	engineered mutation
Leu 228	-	expression tag
Glu 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	100961.8
Non-Polymers*	Number of molecules	31
	Total formula weight	2260.4
All*	Total formula weight	103222.2

*Water molecules are not included.