4ZFJ

Ergothioneine-biosynthetic Ntn hydrolase EgtC, apo form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Amidohydrolase EgtC	polymer	235	25259.0	12	UniProt (A0R5M9) Pfam (PF13230) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
2	I, J, K, L, A...	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	12	Chemie (MES)
3	H, I, J, K, L...	3,6,9,12,15,18,21,24,27-NONAOXANONACOSANE-1,29-DIOL	non-polymer		458.5	11	Chemie (XPE)
4		water	water		18.0	2481	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 227 (UniProt: A0R5M9)

PDB	External Database	Details
Asp 53	Glu 53	engineered mutation
Val 84	Leu 84	engineered mutation
Ser 95	Pro 95	engineered mutation
Ala 118	Asp 118	engineered mutation
Leu 137	Val 137	engineered mutation
Arg 188	His 188	engineered mutation
Ser 228	-	expression tag
Ser 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	303107.7
Non-Polymers*	Number of molecules	23
	Total formula weight	7386.8
All*	Total formula weight	310494.5

*Water molecules are not included.