Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4ZFJ

Ergothioneine-biosynthetic Ntn hydrolase EgtC, apo form

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006541	biological_process	glutamine metabolic process
A	0016787	molecular_function	hydrolase activity
A	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
A	0052699	biological_process	ergothioneine biosynthetic process
A	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
B	0006541	biological_process	glutamine metabolic process
B	0016787	molecular_function	hydrolase activity
B	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
B	0052699	biological_process	ergothioneine biosynthetic process
B	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
C	0006541	biological_process	glutamine metabolic process
C	0016787	molecular_function	hydrolase activity
C	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
C	0052699	biological_process	ergothioneine biosynthetic process
C	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
D	0006541	biological_process	glutamine metabolic process
D	0016787	molecular_function	hydrolase activity
D	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
D	0052699	biological_process	ergothioneine biosynthetic process
D	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
E	0006541	biological_process	glutamine metabolic process
E	0016787	molecular_function	hydrolase activity
E	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
E	0052699	biological_process	ergothioneine biosynthetic process
E	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
F	0006541	biological_process	glutamine metabolic process
F	0016787	molecular_function	hydrolase activity
F	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
F	0052699	biological_process	ergothioneine biosynthetic process
F	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
G	0006541	biological_process	glutamine metabolic process
G	0016787	molecular_function	hydrolase activity
G	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
G	0052699	biological_process	ergothioneine biosynthetic process
G	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
H	0006541	biological_process	glutamine metabolic process
H	0016787	molecular_function	hydrolase activity
H	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
H	0052699	biological_process	ergothioneine biosynthetic process
H	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
I	0006541	biological_process	glutamine metabolic process
I	0016787	molecular_function	hydrolase activity
I	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
I	0052699	biological_process	ergothioneine biosynthetic process
I	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
J	0006541	biological_process	glutamine metabolic process
J	0016787	molecular_function	hydrolase activity
J	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
J	0052699	biological_process	ergothioneine biosynthetic process
J	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
K	0006541	biological_process	glutamine metabolic process
K	0016787	molecular_function	hydrolase activity
K	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
K	0052699	biological_process	ergothioneine biosynthetic process
K	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
L	0006541	biological_process	glutamine metabolic process
L	0016787	molecular_function	hydrolase activity
L	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
L	0052699	biological_process	ergothioneine biosynthetic process
L	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue MES A 301

Chain	Residue
A	LEU39
A	MSE40
A	SER89
A	HOH505
A	HOH547
A	HOH577
C	TYR30
C	TRP66

site_id	AC2
Number of Residues	3
Details	binding site for residue MES A 302

Chain	Residue
C	MSE40
C	SER89
A	TYR30

site_id	AC3
Number of Residues	8
Details	binding site for residue MES B 301

Chain	Residue
B	LEU39
B	MSE40
B	SER89
B	HOH407
B	HOH492
B	HOH566
D	TYR30
D	TRP66

site_id	AC4
Number of Residues	13
Details	binding site for residue XPE B 302

Chain	Residue
A	MSE94
A	SER95
A	HOH475
A	HOH548
B	ALA56
B	ARG58
B	ARG60
B	SER105
B	GLY107
B	HOH482
C	ALA69
C	SER70
C	SER73

site_id	AC5
Number of Residues	13
Details	binding site for residue XPE C 301

Chain	Residue
B	TRP66
B	GLY67
B	ALA69
B	SER70
C	ALA56
C	ARG58
C	ARG60
C	SER99
C	HOH466
C	HOH554
D	GLY93
D	MSE94
D	SER95

site_id	AC6
Number of Residues	8
Details	binding site for residue MES D 301

Chain	Residue
B	TYR30
B	TRP66
D	LEU39
D	MSE40
D	SER89
D	ILE92
D	HOH469
D	HOH538

site_id	AC7
Number of Residues	15
Details	binding site for residue XPE D 302

Chain	Residue
A	ALA56
A	ARG58
A	ARG60
A	PRO98
A	HOH512
B	GLY93
B	MSE94
B	SER95
B	HOH526
D	TRP66
D	GLY67
D	ALA69
D	SER70
D	HOH495
D	HOH505

site_id	AC8
Number of Residues	12
Details	binding site for residue XPE D 303

Chain	Residue
A	TRP66
A	GLY67
A	ALA69
A	SER70
A	ALA72
A	HOH473
C	MSE94
D	ALA56
D	ARG58
D	ARG60
D	HOH501
D	HOH549

site_id	AC9
Number of Residues	6
Details	binding site for residue MES E 301

Chain	Residue
E	LEU39
E	MSE40
E	SER89
E	HOH445
K	TYR30
K	TRP66

site_id	AD1
Number of Residues	17
Details	binding site for residue XPE E 302

Chain	Residue
J	SER70
J	SER73
J	HOH414
J	HOH474
E	ALA56
E	ARG58
E	ARG60
E	PRO98
E	HOH414
H	GLY93
H	MSE94
H	SER95
H	HOH430
H	HOH556
J	TRP66
J	GLY67
J	ALA69

site_id	AD2
Number of Residues	4
Details	binding site for residue MES F 301

Chain	Residue
F	MSE40
F	SER89
L	TYR30
L	TRP66

site_id	AD3
Number of Residues	5
Details	binding site for residue MES G 301

Chain	Residue
G	MSE40
G	SER89
G	HOH404
I	TYR30
I	TRP66

site_id	AD4
Number of Residues	13
Details	binding site for residue XPE G 302

Chain	Residue
F	GLY93
F	MSE94
F	SER95
G	ALA56
G	ARG58
G	ARG60
G	SER105
G	HOH531
L	GLY67
L	ALA69
L	SER70
L	SER73
L	HOH438

site_id	AD5
Number of Residues	5
Details	binding site for residue MES H 301

Chain	Residue
H	LEU39
H	MSE40
H	SER89
H	HOH479
J	TRP66

site_id	AD6
Number of Residues	13
Details	binding site for residue XPE H 302

Chain	Residue
E	MSE94
E	HOH504
H	ALA56
H	ARG58
H	ARG60
H	SER105
H	HOH581
K	GLY67
K	ALA69
K	SER70
K	SER73
K	HOH430
K	HOH489

site_id	AD7
Number of Residues	9
Details	binding site for residue MES I 301

Chain	Residue
G	TYR30
G	TRP66
I	GLY38
I	LEU39
I	MSE40
I	SER89
I	ILE92
I	HOH524
I	HOH550

site_id	AD8
Number of Residues	13
Details	binding site for residue XPE I 302

Chain	Residue
F	TRP66
F	GLY67
F	ALA69
F	SER70
F	ALA72
I	ALA56
I	ARG58
I	ARG60
I	PRO98
I	HOH441
I	HOH595
L	MSE94
L	HOH505

site_id	AD9
Number of Residues	5
Details	binding site for residue MES J 301

Chain	Residue
H	TRP66
J	LEU39
J	MSE40
J	SER89
J	HOH513

site_id	AE1
Number of Residues	10
Details	binding site for residue XPE J 302

Chain	Residue
E	TRP66
E	GLY67
E	ALA69
E	SER70
E	SER73
E	HOH428
J	ARG58
J	ARG60
J	HOH551
K	MSE94

site_id	AE2
Number of Residues	6
Details	binding site for residue MES K 301

Chain	Residue
E	TYR30
E	TRP66
K	LEU39
K	MSE40
K	SER89
K	HOH425

site_id	AE3
Number of Residues	13
Details	binding site for residue XPE K 302

Chain	Residue
H	TRP66
H	GLY67
H	ALA69
H	SER70
H	ALA72
H	HOH437
J	MSE94
K	ARG58
K	ARG60
K	PRO98
K	HOH508
K	HOH529
K	HOH540

site_id	AE4
Number of Residues	8
Details	binding site for residue MES L 301

Chain	Residue
F	TYR30
F	TRP66
L	LEU39
L	MSE40
L	SER89
L	HOH468
L	HOH485
L	HOH525

site_id	AE5
Number of Residues	12
Details	binding site for residue XPE L 302

Chain	Residue
G	TRP66
G	GLY67
G	ALA69
G	SER70
I	MSE94
I	SER95
L	ALA56
L	ARG58
L	ARG60
L	SER105
L	HOH473
L	HOH475

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Nucleophile => ECO:0000255\|PROSITE-ProRule:PRU00609

Chain	Residue	Details
A	CYS2
J	CYS2
K	CYS2
L	CYS2
B	CYS2
C	CYS2
D	CYS2
E	CYS2
F	CYS2
G	CYS2
H	CYS2
I	CYS2

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)