4ZDR

Crystal structure of 14-3-3[zeta]-LKB1 fusion protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	14-3-3 protein zeta/delta,GGSGGS linker,Serine/threonine-protein kinase STK11	polymer	266	30349.3	2	UniProt (P63104) UniProt (Q15831) Pfam (PF00244)	Homo sapiens (Human)	Protein kinase C inhibitor protein 1,KCIP-1,Liver kinase B1,hLKB1,Renal carcinoma antigen NY-REN-19
2	C, D, J (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	E, K (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	F, G, H, I, L... (A, B)	PROPANE	non-polymer		44.1	6	Chemie (TME)
5	N, O (A, B)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, B: 1 - 230 (UniProt: P63104)

PDB	External Database	Details
Met -21	-	expression tag
Ser -20	-	expression tag
Tyr -19	-	expression tag
Tyr -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Ser -9	-	expression tag
Thr -8	-	expression tag
Ser -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Lys -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Leu 0	-	expression tag

A, B: 231 - 236 (PDB: 4ZDR)
A, B: 237 - 244 (UniProt: Q15831)

PDB	External Database	Details
Glu 240	Thr 336	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60698.5
Non-Polymers*	Number of molecules	11
	Total formula weight	733.0
All*	Total formula weight	61431.5

*Water molecules are not included.