4Z8L

Crystal structure of DCAF1/SIV-MND VPX/MND SAMHD1 NTD ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Protein VPRBP	polymer	349	39777.7	2	UniProt (Q9Y4B6)	Homo sapiens (Human)	DDB1- and CUL4-associated factor 1,HIV-1 Vpr-binding protein,VprBP,Serine/threonine-protein kinase VPRBP,Vpr-interacting protein
2	B, E (B, E)	Vpx protein	polymer	100	12204.8	2	UniProt (Q7ZB17) Pfam (PF00522)	Simian immunodeficiency virus (SIV)
3	C, F (C, F)	SAM domain and HD domain-containing protein	polymer	118	13197.7	2	UniProt (H6WEA4) Pfam (PF07647)	Mandrillus sphinx (Mandrill)	SAM domain and HD domain-containing protein 1
4	G, H (B, E)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	I, J, K, L, M... (A, B, C, D, E...)	water	water		18.0	114	Chemie (HOH)

Sequence modifications

A, D: 1057 - 1396 (UniProt: Q9Y4B6)

PDB	External Database	Details
Ala 1056	-	expression tag
Leu 1397	-	expression tag
Glu 1398	-	expression tag
His 1399	-	expression tag
His 1400	-	expression tag
His 1401	-	expression tag
His 1402	-	expression tag
His 1403	-	expression tag
His 1404	-	expression tag

B, E: 4 - 92 (UniProt: Q7ZB17)

PDB	External Database	Details
Ser 1	-	expression tag
Glu 2	-	expression tag
Phe 3	-	expression tag
Leu 93	-	expression tag
Glu 94	-	expression tag
His 95	-	expression tag
His 96	-	expression tag
His 97	-	expression tag
His 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag

C, F: 1 - 115 (UniProt: H6WEA4)

PDB	External Database	Details
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	130360.4
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	130491.2

*Water molecules are not included.