4Z44

F454K Mutant of Tryptophan 7-halogenase PrnA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Flavin-dependent tryptophan halogenase PrnA	polymer	554	62937.1	1	UniProt (P95480) Pfam (PF04820)	Pseudomonas fluorescens
2	B (A)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	E, F, G (A)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	H (A)	water	water		18.0	328	Chemie (HOH)

Sequence modifications

A: 1 - 538 (UniProt: P95480)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Lys 454	Phe 454	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	62937.1
Non-Polymers*	Number of molecules	6
	Total formula weight	1204.2
All*	Total formula weight	64141.2

*Water molecules are not included.