4Z1M

Bovine F1-ATPase inhibited by three copies of the inhibitor protein IF1 crystallised in the presence of thiophosphate.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	ATP synthase subunit alpha, mitochondrial	polymer	510	55302.2	3	UniProt (P19483) Pfam (PF02874) Pfam (PF00006) Pfam (PF00306)	Bos taurus (Bovine)
2	D, E, F (D, E, F)	ATP synthase subunit beta, mitochondrial	polymer	482	51757.8	3	UniProt (P00829) Pfam (PF02874) Pfam (PF00006) Pfam (PF22919)	Bos taurus (Bovine)
3	G (G)	ATP synthase subunit gamma, mitochondrial	polymer	273	30300.8	1	UniProt (P05631) Pfam (PF00231)	Bos taurus (Bovine)	F-ATPase gamma subunit
4	H, I, J (H, I, J)	ATPase inhibitor, mitochondrial	polymer	66	7404.0	3	UniProt (P01096) Pfam (PF04568)	Bos taurus (Bovine)	Inhibitor of F(1)F(o)-ATPase,IF1
5	K, N, R (A, B, C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)
6	CA, L, O, S, W (F, A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
7	M, Q (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	AA, DA, EA, FA, GA... (E, F, G, B, C)	CHLORIDE ION	non-polymer		35.5	11	Chemie (CL)
9	BA, V (F, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
10	HA, IA, JA, KA, LA... (A, B, C, D, E...)	water	water		18.0	25	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 510 (UniProt: P19483)

PDB	External Database	Details
Glu 1	Gln 44	variant
Gly 481	Ser 524	variant

H, I, J: 1 - 60 (UniProt: P01096)

PDB	External Database	Details
Ala 39	Lys 64	engineered mutation
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	373692.8
Non-Polymers*	Number of molecules	23
	Total formula weight	3071.6
All*	Total formula weight	376764.5

*Water molecules are not included.