4YXM

Structure of Thermotoga maritima DisA D75N mutant with reaction product c-di-AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA integrity scanning protein DisA	polymer	377	42763.0	2	UniProt (Q9WY43) Pfam (PF02457) Pfam (PF10635) Pfam (PF00633) In PDB	Thermotoga maritima	Cyclic di-AMP synthase,c-di-AMP synthase,Diadenylate cyclase
2	B	(2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8 ]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide	non-polymer		658.4	1	Chemie (2BA)
3	B	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
4		water	water		18.0	143	Chemie (HOH)

Sequence modifications

A, B: 1 - 357 (UniProt: Q9WY43)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 75	Asp 75	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85526.0
Non-Polymers*	Number of molecules	2
	Total formula weight	776.6
All*	Total formula weight	86302.6

*Water molecules are not included.