4YVK

Crystal Structure of H. influenzae TrmD in complex with sinefungin and tRNA variant (G36C)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA (guanine-N(1)-)-methyltransferase	polymer	266	29750.0	2	UniProt (P43912) Pfam (PF01746)	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	M1G-methyltransferase,tRNA [GM37] methyltransferase
2	C (C)	tRNA	polymer	74	23818.1	1	GenBank (12057205)	Thermotoga maritima MSB8
3	D, E (A, B)	SINEFUNGIN	non-polymer		381.4	2	Chemie (SFG) PubChem (456189) PubChem (4065234) PubChem (6917665) PubChem (12771337) PubChem (25246236) PubChem (176612030) PubChem (177116271) PubChem (46875078) PubChem (46936257) PubChem (65482) PubChem (70789212) PubChem (93287274) PubChem (12771338) PubChem (70602121) PubChem (92403972) PubChem (93287270) PubChem (93287272) PubChem (93475589) PubChem (131971033) PubChem (131972810) PubChem (138626357) PubChem (156562738) PubChem (162866891) PubChem (163339130) PubChem (168280605)

Sequence modifications

A, B: 1 - 246 (UniProt: P43912)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

C: 1 - 76 (GenBank: 12057205)

PDB	External Database	Details
C 36	G 419304	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	83318.2
Non-Polymers*	Number of molecules	2
	Total formula weight	762.8
All*	Total formula weight	84080.9

*Water molecules are not included.